Confluency Assay

Automatic confluency determination using bright field imaging

Automated measurement of confluency using detection of area covered by cells and calculate the fraction of this area from the total scanned area.

  • Determine  cell confluency in each well using brightfield imaging
  • This mode does not require any stain for the nuclei or cytoplasm of cells and can therefore offer a cost and time saving advantage
  • It also avoids the toxicity associated with the use of fluorescent stains over long incubation periods.

Brightfield imaging using 10x objective

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