- Live vs. dead cells can readily be distinguished in images using live/dead cell stains
- Two-colour Images are easily acquired and analyzed using the Athena live/dead application.
- Fast and reliable method, enabling high throughput cytotoxicity assays
- Results are readily visualized as informative graphics
The drug discovery field commonly uses the toxicology assay to evaluate drug candidates. The Live/Dead application demonstrates sub-population identification and analysis. The assay serves to assess cell viability in the presence of various compounds and their combinations, which the live/dead assay can quantify. WiScan Hermes High Content imaging system applies quantitative cytometry and population tools to enable quick and reliable toxicology measurements at high capacities, and the results are readily visualized as informative graphics.
Toxicology assay applied for drug toxicity studies in Cancer research
Hermes data obtained at Virginia Commonwealth University, VA, USA
This paper showed that drug combination leads to a better effect on cell death then treatment with each drug separately.
Drug A (PDES) inhibition with drug B (OSU-03012) activate the death receptor CD95 and lead to cell death in higher efficiency than each drug separately.
The results suggest involvement of ER stress in the drug mechanism of action.
Regulation of OSU-03012 Toxicity by ER Stress Proteins and ER Stress–Inducing Drugs
Booth L. et al.; Mol Cancer Ther, October 2014, 13; 2384