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Mitochondria analysis

Detection & quantification of Mitochondria and their cellular distribution

  • Mitochondria and other  sub-cellular organelles can be detected and analysed using fluorescence labeling.
  • WiSoft Athena‘s automatic and accurate segmentation enables acquiring data for each mitochondria.
  • Various features can be measured, based on fluorescence intensity and morphological features.
  • Option for time lapse imaging and analysis. Press here for more information.

 

 Assay Groups

  • Mitochondrial dynamics studies
  • Mitochondrial numbers and volume measurement
  • Study related human diseases (e.g. Parkinson, Huntington’s disease)

Live imaging of Mitochondria clusters

Time lapse imaging applied with Mito-tracker labeling to follow formation of mitochondria clusters in living cells

 

Mitochondria Morphology 

Captured with 40X/0.75 magnification

Dyes:Red – TMRM (mitochondrial potential marker), green – MitoTracker (mitochondrial marker), blue – Hoechst

Analysis – morphological features were recorded for each mitochondria- length, area, intensity, etc

Mitochondria Morphology -TMRM with segmentation masks

Captured with 40X/0.75NA magnification

Dyes:Red – TMRM (mitochondrial potential marker), green – MitoTracker (mitochondrial marker), blue – Hoechst

Analysis – morphological features were recorded for each mitochondria- length, area, intensity, etc

Detection of Mitochondrion structures was done using WiSoft Minerva dedicated segmentation algorithm . Morphological and fluorescent intensity features of each mitochondrion was calculated and plotted or statistically analyzed to compare different treatments applied on the cells.

 

Mitochondrial Potential

Captured with 20X/0.75NA magnification

Dyes: Red – TMRM (mitochondrial potential marker), green – MitoTracker (mitochondrial marker), blue – Hoechst

Analysis – Potential intensitymarker intensity (red/green intensity ratio)

For more information please contact us at info@idea-bio.com