Pairing a cell-based reporter gene assay with our Hermes high-content screening platform provides the complete solution for a successful screening campaign for translocation of proteins from one cell compartment to another.
- High-throughput acquisition without compromising image quality
- Unique statistical approach for robust measurements
- Automatically detect each cell and nucleus and measure the fluorescence intensity ratio between these two compartments
- Users can obtain quantitative results during image acquisition to save both time and labour
Protein translocation between cellular compartments is a key process in cell biology. A large variety of processes involve translocation, including signalling to modify cell function (e.g. gene expression), immune stimulation in viral infections and cargo transfer to and from the nucleus.
Imaging is a valuable approach to monitor the translocation process by visualizing the protein in the different compartments, measure its intensity and calculate the ratio of intensity of a labelled protein between the cytoplasmic and nuclear compartments as a quantitative measure of translocation.
Nuclear Translocation assay using DAPI + FAR RED labeling and 10X magnification
Automated image analysis, performed simultaneously with acquisition, identifies wells demonstrating high nuclear translocation upon treatment (red), in comparison to the low translocation measured in control wells (blue).