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Imaging Cytometry

Quantitative analysis of multicolor labeled cells

Image Based Cytometry, Sub-Population Identification & Multi-Parametric Analysis
  • Segregate a heterogeneous mixture of cells into a variety of sub-populations as in FACS (fluorescence-activated cell sorting)
  • Automated classification of color-based cellular sub-populations for each well of a micro-well plate
  • Defining morphological features for each sub-population, including intracellular structures, using the high-resolution captured images
  • Suitable for live cell imaging – possibility to follow  and  to return to the sample over time to study changes in each sub-population.
  • Multiple visualization tools to present sub populations

Fluorescent in situ hybridization (FISH)

Quantitative cytometry and population tools applied to detect cells expressing XY chromosome combination.

Y Chromosome (red)
X Chromosome (green)
XY cells – mail (orange mask)
Not XY cells – (blue mask)

FISH assay

 

RNA FISH

RNA FISH (20x magnification) in macrophages stimulated with innate immune agonists.

Nuclei were stained with DAPI (blue) while IL-10 (green) and TNFα (red) were stained by ViewRNA FISH.

 

 

Data courtesy of David Stirling and Matthew Solomons (Noursadeghi Lab, Division of Infection and Immunity, University College London)

For more information please contact us at info@idea-bio.com