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Imaging Cytometry

Quantitative analysis of multicolor labeled cells

Image Based Cytometry, Sub-Population Identification & Multi-Parametric Analysis
  • Segregate a heterogeneous mixture of cells into a variety of sub-populations as in FACS (fluorescence-activated cell sorting)
  • Automated classification of color-based cellular sub-populations for each well of a micro-well plate
  • Defining morphological features for each sub-population, including intracellular structures, using the high-resolution captured images
  • Suitable for live cell imaging – possibility to follow  and  to return to the sample over time to study changes in each sub-population.
  • Multiple visualization tools to present sub populations

Fluorescent in situ hybridization (FISH)

Quantitative cytometry and population tools applied to detect cells expressing XY chromosome combination.

Y Chromosome (red)
X Chromosome (green)
XY cells – mail (orange mask)
Not XY cells – (blue mask)

FISH assay



RNA FISH (20x magnification) in macrophages stimulated with innate immune agonists.

Nuclei were stained with DAPI (blue) while IL-10 (green) and TNFα (red) were stained by ViewRNA FISH.



Data courtesy of David Stirling and Matthew Solomons (Noursadeghi Lab, Division of Infection and Immunity, University College London)

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