Phagokinetic migration screen


Naffar-Abu-Amara et al., PLoS ONE 3(1): e1457 

 

To explore genes that can regulate cell migration, non-motile breast epithelium cells (MCF-7) were transfected with cDNAs encoding a variety of genes associated with breast cancer. Cell migration was then assessed using a phagokinetic track assay, monitored and quantified by automated microscopy. In that assay, tracks depicting cell migration were quantified for their overall area, persistence, roughness and other morphological features. Based on this screen, several "pro-migratory" genes were isolated and subjected to in-depth characterization (e.g. PKC?).


Phagokinetic migration screen

A montage showing cell tracks produced by GFP-MCF7 control cells and images of tracks produced by MCF7 cells expressing the HOXB7, MFGE8, PKC?, FGF7, and ERBB3 genes.
Magnification: 10x. Scale bar: 250 µm.