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WiSoft® Athena Ready-Made Analysis Apps SW

 

Rich and simple application-based analysis software for easy and quick evaluation of experimental results.
Performs common and advanced cell biology applications at the push-of-a-button. 

Intuitive, Powerful, Precise
A software tool for application-derived analysis and visualization of image-based experiments.

Analysis platform for the most common cell biology applications
Athena Incorporates embedded analysis algorithms, statistical evaluation tools and sub-population analysis tools, based on advanced cell quantification tools for measuring fluorescence intensity and morphological features.
Athena consists of a library of pre-built analysis modules applied to data sets of both labeled and non-labeled cells, thus Athena is capable of analyzing images acquired in bright field and fluorescence illumination microscopy.

All level users can be easily trained and use this platform simply and independently
Athena offers a bank of ready-made applications which are loaded by the push of a button and allow the user to start analysis within a few minutes, after performing mild parameter calibrations. The UI and UX are optimized for analyzing image-based cell biology experiments. New comers to high content image analysis can get productive right away using Athena’s pre-configured templates for the most common cell biology applications.

Analysis can be performed on a cell-by-cell basis and at population level
Individual cells can be measured & quantified both for single cell analysis and for subpopulation analysis. Athena contains a population tool which in similarity with cytometry experiments, can be used to define sub-populations of cells in each well and quantify properties of this sub-population based on cellular phenotypes.

• Image acquisition and image analysis can be done simultaneously, independently & at separate locations
View and analyze data from any computer that has an installation of the Athena software, freeing up the Hermes imager for others to use. Experiment time is significantly reduced as images acquired in Hermes system are being uploaded in real-time to a shared storage, from which Athena software extracts data for analysis, in parallel to the image acquisition process.
Experiment metadata, such as filters, objectives, plate type, fields & coverage, time points, z-stack or exposure, are stored in the database for future reference.

Straightforward, simple visual results display
Athena software offers various visualization tools to display the analysis results. At the end of each analysis process, the user can quickly & easily create a summary report summarizing the analysis parameters and presenting the results. Raw data can be easily exported as an excel file to external sources if necessary.

Athena software analysis workflow:

Basic Applications

Application Name Description Color Channels Biological Assays Metrics quantified
Cell Count Count fluorescently labeled cells or objects 1 Color
  • Nucleus
Autophagy

Apoptosis

Cytotoxicity

Cell Viability

Cellular proliferation

Growth rate

Viral plaque assay

Area

Fluorescence intensity

Cell Morphology Fluorescently labeled cells or objects shape quantification

Ideal for assessment of cellular heath

1 Color
  • Cytoplasm
Endocytosis

Phagocytosis

Apoptosis

Cell adherence

Cell migration

Cell viability

Toxicology

Sub-cellular features quantification:
  • Nuclei
  • Actin
  • Microtubules
  • Golgi
  • Vesicles
  • Focal adhesions
  • Mitochondria
Area

Perimeter

Shape metrics:

  • Long/short axis length
  • Elongation ratio
  • Convex Hull (CH) area
  • Solidity
Protein Expression Single-cell fluorescence intensity quantification 3 Colors
  • Nucleus
  • Cytoplasm
  • Protein of interest
Cell Health assessment

Protein degradation

Protein Localization

Protein translocation

Intracellular distribution analysis

Autophagy (t1/2) assays

Ubiquitination

Protein Aggregation &  Accumulation

Transfection efficiency

Gene therapy

Pharmacology & pharmaco-kinetics

Area

Perimeter

Fluorescence intensity

Cell Cycle Determine cell cycle populations using quantitative DNA labeling. 1 Color
  • Nucleus
Proliferation assays

Cell cycle arrest detection

Cytotoxicity

Cell Viability

Area

Fluorescence intensity

Translocation Quantifies the intensity ratio between the sub-cellular compartments 3 Colors
  • Nucleus
  • Cytoplasm
  • Protein of interest
Nuclear signaling assays

Innate immune response

Intra-organelle localization

Intracellular distribution analysis

Fluorescence intensity Nucleus/Cytoplasm Intensity ratio

Specialized Applications

Application name

Description Color channels Biological assays

Metrics quantified

Intracellular Granules Quantify cytoplasmic granules for shape, intensity and number 3 Colors
  • Nucleus
  • Cytoplasm
  • Granule channel
Protein aggregation

Vesicle trafficking

Puncta analysis

Endo/exocytosis

Receptor or protein clustering

Granule count

Granule intensity

Granule area & shape

Cell Count in Colonies Colony count estimation extrapolated from single cell fluorescence intensities 1 Color
  • Nucleus or cell
Bacterial colony cell count Small, high density cellular colonies
e.g. Embryonic stem cells
Populations:
  • Single cells
  • Colonies

Morphology

Fluorescence intensity

Intranuclear Foci Detection and quantification of intranuclear foci and puncta 3 Colors
  • Nucleus
  • Cytoplasm
  • Foci channel
Intranuclear protein aggregation

Nucleolar detection

Transcription factor detection

Chromatin detection

Nuclear area

Foci count

Foci intensity

Foci area & shape

Quantitative Cytometry Multiplex quantitation of biomarkers or objects within cells or nuclei 1-7 Colors
  • Nucleus or cell
  • 1 color for each object of interest
Cytotoxicity

Autophagy

Apoptosis

Cellular fusion

Multi-plasmid transfection

Live/Dead measurement

Bacterial viability

Multi-color foci/puncta

Count per object

Intensity per object

Live-Dead Toxicology Quantify live vs. dead cells using cell viability markers 2 Colors
  • Live cells
  • Dead cells
Cytotoxicity

Toxicology

Cell viability

Pharmacology & pharmacokinetics Live cells count

Dead cell count

Spheroid Morphology Morphology and fluorescence intensity quantification for 3D cell culture 1 Color
  • Nuclei or Brightfield
Drug dose response curves

Cytotoxcitity

Toxicology

Growth curves

Pharmacology & pharmacokinetics

Area

Perimeter

Morphology

Solidity

Fluorescence intensity

Colony Detection Label-free cell colony detection providing true single-cell count per colony Brightfield Clonogenic assay

Radiotherapy assay

Dose response curve

Cell viability & proliferation

Pharmacology & pharmacokinetics

Colony metrics:
  • Single cell counting
  • Size
  • Morphology
Yeast Quantification Brightfield yeast cell segmentation & counting Brightfield Cell replication & proliferation

Genetic profiling

RNA colocalization with intracellular organelle in yeast Count colocalized

Count close distance events

Number of RNA spots per cell

Number of organelle per cell

 

Soon to be released

Application Name Description Color Channels Biological Assays Metrics quantified
Fiber Detection Detect fiber and cytoskeletal intra-cellular structure

*Released Soon*

1 Color
  • Fibers
Cytoskeletal rearrangement Fiber length

Count per cell

Fluorescence intensity

Confluency Label-free measure of the area fraction covered by cells or colonies

*Released Soon*

Brightfield Cell viability & proliferation

Radiotherapy assay

Growth rates

Population pharmacology & pharmacokinetics

Confluency

Total cell area

Mitochondria Quantification Quantification of mitochondria distribution, morphology and intensity

*Released Soon*

3 Colors
  • Nucleus
  • Cytoplasm
  • Mitochondria
Cellular metabolism

Cell health and viability

Mitochondrial dynamics:
  • Number
  • Size
  • Distribution
Mitochondria count per cell

Intensity of mitochondria

Morphological properties

Distance from nucleus

Cell Count in Brightfield Label-free cell counting in adherent cell culture

*Released Soon*

Brightfield Cell viability & proliferation

Radiotherapy assay

Growth rates

Pharmacology & pharmacokinetics

Count

Cell area

Zebra Fish Detect fluorescent structures or objects within Zebrafish

*Released Soon*

2 Colors
  • Brightfield
  • Fluorescent object
 Bacterial infection  Metastasis growth assay
 C. elegans  Detect fluorescent structures or objects within
C. elegans*Released Soon*
2 Colors
  • Brightfield
  • Fluorescent object
 Cell type identification
 Scratch Assay Detect scratch edge to measure the scratch area relative to total

*Released Soon*

2 Colors
  • Brightfield
  • Fluorescent object
 Wound healing scratch assay

 

 

• Windows 7 or Windows 10 required
• Athena Software runs under 32-bit and 64-bit operating systems.
• Results report produced in PDF format
• Raw data can be exported in CSV format to Excel

 

Typical size and storage

Size of typical sample:

Images – TIF format 1-10 GB
Masks – special format – 1 MB
Analyzed data – CSV files – 1-10 KB

Storage of typical sample:

  • Data set including reports
  • Images in  well folders

 Brochure

Application Notes