Automated measurement of confluency using detection of area covered by cells and calculate the fraction of this area from the total scanned area.
- Determine cell confluency in each well using brightfield imaging
- This mode does not require any stain for the nuclei or cytoplasm of cells and can therefore offer a cost and time saving advantage
- It also avoids the toxicity associated with the use of fluorescent stains over long incubation periods.
Brightfield imaging using 10x objective