- Rapid automated and Manual fish embryo and internal organelles detection
- Quantify area, fluorescence intensity, and count of whole fish and internal organelle properties, including eye, yolk, spine, tail, brain, granules and more
- Label free or fluorescently tagged fish and internal organelles may be analyzed
- Automatic segmentation or manual definition of multiple fish organelles
- Analysis of images acquired in single plane, Z stack and projections
- Statistical data calculated per fish and per organelle
Live Zebrafish imaging- Blood flow
Video capture from a live Zebrafish larva imaged in bright field illumination using 40X magnification. Acquired by Dr Gillian Tomlinson using IDEA Bio-Medical’s Hermes WiScan at the UCL Division of Infection and Immunity, London, UK.
The zebrafish (Danio rerio) has become a popular in vivo model of host-pathogen interactions due to its transparent larval stage that is particularly well suited for imaging .
Sample throughput remains an important challenge in the field due to the relatively large specimen size compared to 2D cell culture and the need to immobilize the fish for imaging. This unique analysis application enables automated quantification of Zebrafish embryos and their internal organelle properties, including eye, yolk, spine, tail, brain, granules and more, thus significantly enhancing analysis rate compared to conventional, manual acquisition and measurements.
Zebrafish embryo injected IV with FITC-dextran (green) and captured with Hermes imaging system using 4X magnification