Brighter images, Higher resolution
Fast, automated imaging with Oil immersion objectives
Oil Vs. Air objectives: shorter illumination times, brighter images & enhanced resolution.
Same sample of Yeast obtained at 2 different 60X magnifications. Same image contrast is achieved in both.
However, with 60X/1.4NA oil immersion objective, we reach 60% shorter illumination times, brighter images and enhanced resolution.
With IDEA Bio-Medical’s NEW precision oil dispensing unit on Hermes imaging system, you can now quickly & easily get such images in fully autonomous plate scanning.
Why use High NA Oil Objectives in High Content Screening?
☑ Shorter Exposure
☑ Brighter Images
☑ Higher Resolution
Oil immersion objectives in automated microscopy? – Now you can!
Up until now, oil immersion objectives have been off-limits for automated microscopy, and for high-content screening in particular, because of the required user intervention to add oil while scanning a sample. Additionally, just the right amount of oil must remain between the objective and the sample when automatically scanning across it. Lastly, the index of refraction matching that enhances image resolution and brightness makes autofocusing more challenging.
We at IDEA Bio-Medical recognized these challenges to automated microscopy, but also the benefits that it could bring to the life-sciences research community. Relying on our decades-long experience in many fields of ODM & OEM engineering, we have created a unique and fully automated technology that precisely adds immersion oil only when needed. Such precision prevents excess oil from collecting and dripping from the bottom of a sample, as well as a lack of oil during a scan.
Our autofocus mechanism is fully compatible with immersion oil, enabling clear and robust imaging. And cleaning is a snap, thanks to our quick-snap objective exchange technology, making it easy to take objectives out of the microscope.
Now, for the first time, an high-content imaging system can utilize oil immersion objectives for automating high resolution cell biology applications. Samples in a multi-well plate format can be scanned in their entirety, unsupervised and with no user intervention. The system can accommodate a variety of multi-well plates and sample formats and offers environmental control for live cell assays.
Utilizing high NA oil objectives also enables fully automated super-resolution imaging.
Full automation, No Compromises
☑ Unique hardware automatically adds immersion oil to objectives
☑ No user intervention; No oil spilling
☑ Autonomous, rapid image acquisition for:
– Full-plate scanning
– Time-lapse imaging of live cells
☑ Optimized autofocus, X, Y, Z motion and long-duration oil capsules for easy maintenance
- Super-resolution radial fluctuations (SRRF) live-cell imaging
- Fluorescence In-Situ Hybridization (FISH)
- Microbiology, Virology & Yeast studies
- Spot / Foci / Granule visualization
- Mitochondria, Endoplasmic Reticulum, Cytoskeleton & Focal Adhesion imaging
Selectable oil immersion objectives:
Magnification | NA | Working distance (um) |
100X | 1.45 | 130 |
60X | 1.42 | 150 |
40X | 1.4 | 130 |
Blog post: How oil immersion objectives can improve your microscopy?
