Home > All Applications > Imaging Cytometry
Imaging Cytometry Assay
Quantitative analysis of multicolor (multiplexed) labeled cells
- Image-Based Cytometry, Sub-Population Identification & Multi-Parametric Analysis
Segregate a heterogeneous mixture of cells into a variety of sub-populations as in FACS (fluorescence-activated cell sorting). - Automated classification of color-based cellular sub-populations for each well of a micro-well plate
- Defining morphological features for each sub-population, including intracellular structures, using the high-resolution captured images
- Suitable for live cell imaging – possibility to follow and to return to the sample over time to study changes in each sub-population.
- Multiple visualization tools to present sub populations
Fluorescent in situ hybridization (FISH)
Quantitative cytometry and population tools applied to detect cells expressing XY chromosome combination.
Y Chromosome (red) X Chromosome (green) XY cells – mail (orange mask) Not XY cells – (blue mask)
RNA FISH
RNA FISH (20x magnification) in macrophages stimulated with innate immune agonists.
Nuclei were stained with DAPI (blue) while IL-10 (green) and TNFα (red) were stained by ViewRNA FISH.
Data courtesy of David Stirling and Matthew Solomons (Noursadeghi Lab, Division of Infection and Immunity, University College London)
Image Based Cytometry, Sub-Population Identification & Multi-Parametric Analysis
Segregate a heterogeneous mixture of cells into a variety of sub-populations as in FACS (fluorescence-activated cell sorting)
Automated classification of color-based cellular sub-populations for each well of a micro-well plate
Defining morphological features for each sub-population, including intracellular structures, using the high-resolution captured images
Suitable for live cell imaging – possibility to follow and to return to the sample over time to study changes in each sub-population.
Multiple visualization tools to present sub populations
Fluorescent in situ hybridization (FISH)
Quantitative cytometry and population tools applied to detect cells expressing XY chromosome combination.
Y Chromosome (red)
X Chromosome (green)
XY cells – mail (orange mask)
Not XY cells – (blue mask)
RNA FISH
RNA FISH (20x magnification) in macrophages stimulated with innate immune agonists.
Nuclei were stained with DAPI (blue) while IL-10 (green) and TNFα (red) were stained by ViewRNA FISH.
Data courtesy of David Stirling and Matthew Solomons (Noursadeghi Lab, Division of Infection and Immunity, University College London)