Home > All Applications > Intranuclear Foci

Intranuclear Foci Assay

Automatic detection & quantification of intranuclear foci and puncta

This application detects fluorescent foci within cellular nuclei and quantifies their count, area and intensity.

In parallel, the intensity of a labeled protein of interest in the nuclei is measured and quantified as well.

Download App Note

Single cell analysis of RNA FISH for cytokine expression

In this application note, we utilized WiScan® Hermes high content microscope to investigate immune responses to stimuli by primary human monocyte-derived macrophages, which exhibit significant variability in gene expression between different cells. WiScan® Hermes High content wide field microscope offers the ability to work with large sample sizes while investigating subcellular features at the single cell level.