Spheroids Imaging & 3D Imaging
Image & Quantify Spheroids easily, efficiently & always in focus
Spheroids & Live/Dead Detection
Some spheroids permit the visualization of individual cells,enabling single-cell live/dead quantification on a per-spheroid basis.
The ratio of live to dead cells can be obtained directly within the WiSoft Athena software environment and can be normalized to total cell count or live vs. dead.
Spheroid Solidity (S)
Solidity, S, is the measurement of the overall concavity of a particle. As the particle becomes more solid the image area and convex hull area approach each other resulting in a solidity value of one.
However, as the particle form digresses from a closed circle, the convex hull area increases and the calculated solidity decreases. Solidity is a dimensionless value.
Single cell metrics within Spheroids. Cell nuclei labeled with DAPI (Blue) provide total cell count for quantification of calcein-labeled live cells (Green) and PI-labeled (Red) dead cells.
Automated Spheroid analysis workflow. Tumorgenic cells (green) co-cultured with normal tissue-specific fibroblasts (Red).
- Capture properly focused images of spheroids in their ideal growth environment in U-shape bottom plates
- Easily spot Spheroids using unique methodology of rapid scanning for spheroid localization
- Simple and labour reducing automated analysis of spheroid relevant features
- Monitor Spheroid growth over the entire plate using plate view
- Classify Spheroids of specific, desired features using sub-population tool
- Apply Live/ Dead Spheroid Assay to monitor viability of 3D tumour spheroids
- Visualize spheroid morphology over a range of depths using flexible multi-plane definitions
- Monitor Spheroid growth over the entire plate
Live imaging of a Spheroid of bladder cells labeled with DAPI Calcein and PI for nuclei live and dead cells respectively, imaged at 10X magnification over 40 hours time course.
