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Time Lapse Live Cell Assays
Capture time-dependent events using rapid acquisition rate & accurate localization repeatability
Hermes High content imaging system incorporates environmental control to enable live cell experiments with time-lapse imaging to continuously monitor live cell proliferation, morphology and viability, follow dynamic processes and characterize changes of cell populations.
Real-time cell analysis applied with Rare event detection feature can be applied to detect isles of interest within the well and re-visit specific objects to monitor and capture their activity over time.
- Environmental control for Temperature, CO2 and relative humidity. Hypoxia/Hyperoxia conditions optional
- Rapid scanning rate (e.g. 96 locations in less than 2 minutes)
- 200mm localization repeatability
- Microplate does not move along the scanning process, avoiding vibrations to the gentle biological samples and achieving high re-positioning accuracy
- Multi time point scanning
- Flexible definition of cycles and intervals
- Apply real-time cell analysis using rare events automatic detection and follow-up over time with option to select objects for re-visiting
- Spheroid growth assays
Multiwell plate view of spheroid growth assay using time lapse experiment of 40h
Live Zebrafish imaging
Video capture from a live Zebrafish larva imaged in bright field illumination using 10X magnification.
Acquired by Dr Gillian Tomlinson using IDEA Bio-Medical’s Hermes WiScan at the UCL Division of Infection and Immunity, London, UK.
Real time Phagocytosis
HKTB MDM Denoise: Human monocyte derived macrophages with heat killed M tuberculosis (green). With thanks to Dr Meera Mehta, UCL Division of Infection & Immunity
C-Elegans live imaging
Live imaging of the nematode Caenorhabditis Elegans. Acquired with Hermes high content imaging system in bright field and Red fluorescence channel.
Mitochondria in live cells
Mito-tracker labelling to follow the formation of mitochondria clusters in live cells.
Clusters dynamics
OT-II T-cells (blue) co-cultured with ovalbumin-loaded DCs (green), with cell death marker (red) (propidium iodide). Both clusters and single cells were able to join and leave existing clusters.
Dynamics of clusters of T-cells stimulated by antigen-loaded dendritic cells
Shimrit Adutler-Lieber, Nir Friedman, Benjamin Geiger
Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Multiwell plate view of spheroid growth assay using time lapse experiment of 40h
Live Zebrafish imaging
Video capture from a live Zebrafish larva imaged in bright field illumination using 10X magnification.
Acquired by Dr Gillian Tomlinson using IDEA Bio-Medical’s Hermes WiScan at the UCL Division of Infection and Immunity, London, UK.
Real time Phagocytosis
HKTB MDM Denoise: Human monocyte derived macrophages with heat killed M tuberculosis (green). With thanks to Dr Meera Mehta, UCL Division of Infection & Immunity
C-Elegans live imaging
Live imaging of the nematode Caenorhabditis Elegans. Acquired with Hermes high content imaging system in bright field and Red fluorescence channel.
Mitochondria in live cells
Mito-tracker labelling to follow the formation of mitochondria clusters in live cells
.
Clusters dynamics
OT-II T-cells (blue) co-cultured with ovalbumin-loaded DCs (green), with cell death marker (red) (propidium iodide). Both clusters and single cells were able to join and leave existing clusters.
Dynamics of clusters of T-cells stimulated by antigen-loaded dendritic cells
Shimrit Adutler-Lieber, Nir Friedman, Benjamin Geiger
Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
.