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Mitochondria Analysis Assays
Detection & quantification of Mitochondria and their cellular distribution
Mitochondria and other sub-cellular organelles can be detected and analyzed using fluorescence labelling.
WiSoft Athena‘s automatic and accurate segmentation enables acquiring data for each mitochondria.
Various features can be measured, based on fluorescence intensity and morphological features.
Option for time lapse imaging and analysis
- Mitochondrial dynamics studies
- Mitochondrial numbers and volume measurement
- Study related human diseases (e.g. Parkinson, Huntington’s disease)
- Live imaging of Mitochondria clusters
Time lapse imaging applied with Mito-tracker labelling to follow formation of mitochondria clusters in living cells
Mitochondria Morphology
Captured with 40X/0.75 magnification
Dyes:Red – TMRM (mitochondrial potential marker), green – MitoTracker (mitochondrial marker), blue – Hoechst
Analysis – morphological features were recorded for each mitochondria- length, area, intensity, etc
Mitochondrial Potential
Captured with 20X/0.75NA magnification
Dyes: Red – TMRM (mitochondrial potential marker), green – MitoTracker (mitochondrial marker), blue – Hoechst
Analysis – Potential intensitymarker intensity (red/green intensity ratio)
Mitochondria and other sub-cellular organelles can be detected and analysed using fluorescence labelling.
WiSoft Athena‘s automatic and accurate segmentation enables acquiring data for each mitochondria.
Various features can be measured, based on fluorescence intensity and morphological features.
Option for time lapse imaging and analysis
- Mitochondrial dynamics studies
- Mitochondrial numbers and volume measurement
- Study related human diseases (e.g. Parkinson, Huntington’s disease)
- Live imaging of Mitochondria clusters
Time lapse imaging applied with Mito-tracker labelling to follow formation of mitochondria clusters in living cells
Mitochondria Morphology
Captured with 40X/0.75 magnification
Dyes:Red – TMRM (mitochondrial potential marker), green – MitoTracker (mitochondrial marker), blue – Hoechst
Analysis – morphological features were recorded for each mitochondria- length, area, intensity, etc
Mitochondrial Potential
Captured with 20X/0.75NA magnification
Dyes: Red – TMRM (mitochondrial potential marker), green – MitoTracker (mitochondrial marker), blue – Hoechst
Analysis – Potential intensitymarker intensity (red/green intensity ratio)