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Time Lapse Live Cell Assays
Capture time-dependent events using rapid acquisition rate & accurate localization repeatability
Hermes High content imaging system incorporates environmental control to enable live cell experiments with time-lapse imaging to continuously monitor live cell proliferation, morphology and viability, follow dynamic processes and characterize changes of cell populations.
Real-time cell analysis applied with Rare event detection feature can be applied to detect isles of interest within the well and re-visit specific objects to monitor and capture their activity over time.
- Environmental control for Temperature, CO2 and relative humidity. Hypoxia/Hyperoxia conditions optional
- Rapid scanning rate (e.g. 96 locations in less than 2 minutes)
- 200mm localization repeatability
- Microplate does not move along the scanning process, avoiding vibrations to the gentle biological samples and achieving high re-positioning accuracy
- Multi time point scanning
- Flexible definition of cycles and intervals
- Apply real-time cell analysis using rare events automatic detection and follow-up over time with option to select objects for re-visiting
- Spheroid growth assays
Live Zebrafish imaging
Video capture from a live Zebrafish larva imaged in bright field illumination using 10X magnification.
Acquired by Dr Gillian Tomlinson using IDEA Bio-Medical’s Hermes WiScan at the UCL Division of Infection and Immunity, London, UK.
Real time Phagocytosis
HKTB MDM Denoise: Human monocyte derived macrophages with heat killed M tuberculosis (green). With thanks to Dr Meera Mehta, UCL Division of Infection & Immunity
C-Elegans live imaging
Live imaging of the nematode Caenorhabditis Elegans. Acquired with Hermes high content imaging system in bright field and Red fluorescence channel.
Mitochondria in live cells
Mito-tracker labelling to follow the formation of mitochondria clusters in live cells.
Clusters dynamics
OT-II T-cells (blue) co-cultured with ovalbumin-loaded DCs (green), with cell death marker (red) (propidium iodide). Both clusters and single cells were able to join and leave existing clusters.
Dynamics of clusters of T-cells stimulated by antigen-loaded dendritic cells
Shimrit Adutler-Lieber, Nir Friedman, Benjamin Geiger
Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Live Zebrafish imaging
Video capture from a live Zebrafish larva imaged in bright field illumination using 10X magnification.
Acquired by Dr Gillian Tomlinson using IDEA Bio-Medical’s Hermes WiScan at the UCL Division of Infection and Immunity, London, UK.
Real time Phagocytosis
HKTB MDM Denoise: Human monocyte derived macrophages with heat killed M tuberculosis (green). With thanks to Dr Meera Mehta, UCL Division of Infection & Immunity
C-Elegans live imaging
Live imaging of the nematode Caenorhabditis Elegans. Acquired with Hermes high content imaging system in bright field and Red fluorescence channel.
Mitochondria in live cells
Mito-tracker labelling to follow the formation of mitochondria clusters in live cells
.
Clusters dynamics
OT-II T-cells (blue) co-cultured with ovalbumin-loaded DCs (green), with cell death marker (red) (propidium iodide). Both clusters and single cells were able to join and leave existing clusters.
Dynamics of clusters of T-cells stimulated by antigen-loaded dendritic cells
Shimrit Adutler-Lieber, Nir Friedman, Benjamin Geiger
Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
.