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Cell Viability – Toxicology Assay
Fast and reliable high throughput cytotoxicity assays
Toxicology assay applied for drug toxicity studies in Cancer research
Regulation of OSU-03012 Toxicity by ER Stress Proteins and ER Stress–Inducing Drugs; Booth L. et al.; Mol Cancer Ther, October 2014, 13; 2384
This paper showed that drug combination leads to a better effect on cell death then treatment with each drug separately. Drug A (PDES) inhibition with drug B (OSU-03012) activate the death receptor CD95 and lead to cell death in higher efficiency than each drug separately. The results suggest involvement of ER stress in the drug mechanism of action.
Hermes imaging data obtained at Virginia Commonwealth University, VA, USA.
The drug discovery field commonly uses the toxicology assay to evaluate drug candidates. The Live/Dead application demonstrates sub-population identification and analysis. The assay serves to assess cell viability in the presence of various compounds and their combinations, which the live/dead assay can quantify. WiScan Hermes high content imaging system applies quantitative cytometry and population tools to enable quick and reliable toxicology measurements at high capacities, and the results are readily visualized as informative graphics.
- Live vs. dead cells can readily be distinguished in images using live/dead cell stains
- Two-colour Images are easily acquired and analysed using the Athena live/dead application.
- Fast and reliable method, enabling high throughput cytotoxicity assays
- Results are readily visualized as informative graphics
Regulation of OSU-03012 Toxicity by ER Stress Proteins and ER Stress–Inducing Drugs
Booth L. et al.; Mol Cancer Ther, October 2014, 13; 2384
Toxicology assay applied for drug toxicity studies in Cancer research
This paper showed that drug combination leads to a better effect on cell death then treatment with each drug separately. Drug A (PDES) inhibition with drug B (OSU-03012) activate the death receptor CD95 and lead to cell death in higher efficiency than each drug separately. The results suggest involvement of ER stress in the drug mechanism of action.
Hermes data obtained at Virginia Commonwealth University, VA, USA
Live Zebrafish imaging at 10x magnification
Video capture from a live Zebrafish larva
With thanks to Dr Gillian Tomlinson from the UCL Division of Infection and Immunity, UCL, UK
Live Zebrafish imaging- Blood flow
Video capture from a live Zebrafish larva imaged in bright field illumination using 40X magnification. Acquired by Dr Gillian Tomlinson using IDEA Bio-Medical’s Hermes WiScan at the UCL Division of Infection and Immunity, London, UK.
Fish organs & regions automatic segmentation
Automatically quantify area, fluorescence intensity, and count of whole fish and internal organelle properties, including eye, yolk, spine, tail, brain, internal granules and more.Statistical data calculated per fish and per organelle.
Time lapse Zebrafish- Neutrophil Migration
Time lapse of a Zebrafish embryo with S. Pneumoniae injected into the hind brain. GFP-expressing Neutrophils begin to migrate into the injection site over 4 hours.Acquired with IDEA Bio-Medical’s Hermes automated screening system by Sreyashi Koyel Basu and Dr. Gillian Tomlinson, UCL, London, UK
Live Zebrafish imaging at 10x magnification
Video capture from a live Zebrafish larva
With thanks to Dr Gillian Tomlinson from the UCL Division of Infection and Immunity, UCL, UK
Live Zebrafish imaging- Blood flow
Video capture from a live Zebrafish larva imaged in bright field illumination using 40X magnification. Acquired by Dr Gillian Tomlinson using IDEA Bio-Medical’s Hermes WiScan at the UCL Division of Infection and Immunity, London, UK.
Fish organs & regions automatic segmentation
Automatically quantify area, fluorescence intensity, and count of whole fish and internal organelle properties, including eye, yolk, spine, tail, brain, internal granules and more.Statistical data calculated per fish and per organelle.
Time lapse Zebrafish- Neutrophil Migration
Time lapse of a Zebrafish embryo with S. Pneumoniae injected into the hind brain. GFP-expressing Neutrophils begin to migrate into the injection site over 4 hours.Acquired with IDEA Bio-Medical’s Hermes automated screening system by Sreyashi Koyel Basu and Dr. Gillian Tomlinson, UCL, London, UK