WiScan Hermes High Content Imaging System

Versatile
Whether you are performing assay development, compound screens, transfection assays or looking at a few samples in great detail, and whether you are using 3D models (such as spheroids, Organoids), Zebrafish imaging, primary cells, fixed cells or live cell imaging, WiScan® Hermes is the solution for you.

Reliable
WiScan Hermes, IDEA Bio-Medical’s automated imaging system for high content screening (HCS) provides the unique combination of the two contradicting primary functions of automated microscopy: Image quality and acquisition speed.

Robust
WiScan® Hermes’ mechanisms are based on patents, creatively designed to meet heavy duty operation demands (24/7) with full process robustness.

Flexible
WiScan® Hermes is a cost-effective system that is both sophisticated and flexible, offering 7 fluorescence colors, bright field option, and a large range of air objectives. The system can accommodate a variety of multi-well plates and sample formats (slides, dishes…) and offers environmental control for live cell assays.

Intuitive
It doesn’t matter if you are a beginner or an experienced microscopist, Hermes easily allows you to look deeper into your samples. The system is intuitively operated. Its built-in applications are extremely easy to use, and are operated at the push-of-a-button.

Download Hermes Product Family Brochure

Download Hermes Product Brochure

Hermes Real-time Observer data sheet

3D reader: EPI-fluorescence inverted optics mounted on XYZ (patented) linear scanner

• Auto Focus: Patented ultra-fast laser-based Auto Focus with 100nm resolution
• XY motion: Accurate positioning with 200nm repeatability
• Illumination sources: Hermes Real-time observer includes 4 fluorescence channels DAPI,,GFP,,RFP,CY5.
• Up to 7 optional LED sources are available in other Hermes models (DAPI,CFP,GFP,YFP,RFP,mCherry,CY5).
• Transmission: White LED source
• Optical Filters: 2 emission filters and compatible dichroic filters (automatically exchanged)
• Objectives (Air): Choice of air objectives in the range: 2X to 60X, high NA.
• Oil/ Water immersion objectives: Option to add automated immersion media to allow use of immersion objectives (oil/water) – optional hardware upgrade.
• Camera: High sensitivity CMOS camera with 5.1MPixel resolution
• Sample format: Supports full-area screening of 6-1536 well plates Supports slides, microarrays, 35 mm dish formats. U-shaped bottom plats are optional. Flexible and simple interface for introducing new sample formats to the microscope.
• IT: PC with Windows® operating system and touch screen. Joystick for microscope navigation.
• Enclosure: Allows operation in fully lit areas
• Desktop standalone platform: 47 W x 72 D x 57 H (cm), 18.5 W x 28.5 D x 22.4 H (inches) With plate cover closed.
• Certification: CE, UL

• Live cell imaging: Allow long time lapse experiments. Sample does not move during most of the scanning process.
• Live cell conditions
• Temperature control: Ranges from ambient+5°C to 40°C ±0.5°C
• CO2 level control: enables setting of 0.02 L/min- 0.13 L/min mixed with air for desired CO2 percentage. (external accessory). CO2 Range: 0-15%. Set Point Resolution: 1%
• Humidity

• Object mapping for rare events detection
• First scan: rapid scan of entire plate with low magnification, for efficient region of interest object mapping.
• Second scan: re-visitng solely detected objects with high magnification 
• Analysis tools: WiSoft® based image processing tools for object definition and detection in the first low magnification scan and further tools to analyze the detected objects with high magnification
• Objectives: Automatic objective exchanger, accommodates of up to 3 objectives at a time
• Throughput: Enhances the throughput of high magnification scanning of rare objects
• Statistical tools: Provides automatic decision resulting in efficient screening for minimum required objects in a well
• Combination of HCS and HTS: Ultra-fast High Content Screening unit
• Image acquisition speed >10 images per second (depends on experiment conditions)
• Throughput / Acquisition speed: A full 96-well plate screening using 10X magnification with a single field per well, four fluorescence colors, 50ms exposure time per channel, runs in ~2 minutes. With some applications, this will include data processing and analysis results simultaneously with image acquisition.
• Automation Remote accessibility: Protocols ( applications and screening parameters ) execution and management
• External loader compatibility to 3rd-party robotics (Robot/manipulator)
• Communication channel for control by external equipment
• Loading door with automatic opening/closing synchronized with external equipment
• External loader(Robot/manipulator) integration: Full integration supported
  

  Download Hermes Product Family Brochure

  Download Hermes Product Brochure

Illumination Channels Options:

Hermes system incorporates an automated objective exchanger which holds up to 3 objectives at a time.

Hermes supports air objectives and can optionally support a unique immersion media dispensing to allow oil/water immersion objectives.

Objectives selection:

Automated immersion media scanning 

Now, for the first time, a high-content imaging system can utilize oil immersion objectives for automating high resolution cell biology applications. Samples in a multi-well plate format can be scanned in their entirety, unsupervised and with no user intervention. The system can accommodate a variety of multi-well plates and sample formats and offers environmental control for live cell assays.

Utilizing high NA oil objectives also enables fully automated super-resolution imaging. 

Full automation, No Compromises

 Unique hardware automatically adds immersion oil to objectives
 No user intervention; No oil spilling
 Autonomous, rapid image acquisition for:
      – Full-plate scanning
      – Time-lapse imaging of live cells
Optimized autofocus, X, Y, Z motion and long-duration oil capsules for easy maintenance

Flexible plate formats & sample holders

• Supports full-area screening of 6-1536 well plates
• Holders for additional sample formats are available:
• Slide holder
• Chamber holder
• Dish (3cm) holder
• Histology slides, Spotted Array
• Any SBS-standard format can be easily introduced to the Hermes system using a simple definition screen, for maximum flexibility

• Optional sample holders

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